Co-Expression of Foreign Proteins Tethered to HIV-1 Envelope Glycoprotein on the Cell Surface by Introducing an Intervening Second Membrane-Spanning Domain

The envelope glycoprotein (Env) of human immunodeficiency virus type I (HIV-1) mediates membrane fusion. To analyze the mechanism of HIV-1 Env-mediated membrane fusion, it is desirable to determine the expression level of Env on the cell surface. However, the quantification of Env by immunological s...

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Bibliographic Details
Main Authors: Wang, Hongyun, Li, Xiao, Nakane, Shuhei, Liu, Shujun, Ishikawa, Hirohito, Iwamoto, Aikichi, Matsuda, Zene
Format: Artigo
Language:Inglês
Published: Public Library of Science 2014
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4013048/
https://ncbi.nlm.nih.gov/pubmed/24804933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0096790
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