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Sets of Covariant Residues Modulate the Activity and Thermal Stability of GH1 β-Glucosidases

The statistical coupling analysis of 768 β-glucosidases from the GH1 family revealed 23 positions in which the amino acid frequencies are coupled. The roles of these covariant positions in terms of the properties of β-glucosidases were investigated by alanine-screening mutagenesis using the fall arm...

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Detalhes bibliográficos
Main Authors: Tamaki, Fábio K., Textor, Larissa C., Polikarpov, Igor, Marana, Sandro R.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4013033/
https://ncbi.nlm.nih.gov/pubmed/24804841
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0096627
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