Gene replacement in Dictyostelium: generation of myosin null mutants.

The eukaryotic slime mold Dictyostelium discoideum has a single conventional myosin heavy chain gene (mhcA). The elimination of the mhcA gene was achieved by homologous recombination. Two gene replacement plasmids were constructed, each carrying the G418 resistance gene as a selective marker and fla...

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Библиографические подробности

Опубликовано в::	EMBO J
Главные авторы:	Manstein, D J, Titus, M A, De Lozanne, A, Spudich, J A
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Nature Publishing Group 1989
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC400892/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2721503/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb03453.x
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