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Gene replacement in Dictyostelium: generation of myosin null mutants.

The eukaryotic slime mold Dictyostelium discoideum has a single conventional myosin heavy chain gene (mhcA). The elimination of the mhcA gene was achieved by homologous recombination. Two gene replacement plasmids were constructed, each carrying the G418 resistance gene as a selective marker and fla...

詳細記述

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書誌詳細
出版年:EMBO J
主要な著者: Manstein, D J, Titus, M A, De Lozanne, A, Spudich, J A
フォーマット: Artigo
言語:Inglês
出版事項: Nature Publishing Group 1989
主題:
オンライン・アクセス:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC400892/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2721503/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb03453.x
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