Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

BACKGROUND: In Pichia pastoris bioprocess engineering, classic approaches for clone selection and bioprocess optimization at small/micro scale using the promoter of the alcohol oxidase 1 gene (P(AOX1)), induced by methanol, present low reproducibility leading to high time and resource consumption. R...

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Detalhes bibliográficos
Main Authors: Hemmerich, Johannes, Adelantado, Núria, Barrigón, José Manuel, Ponte, Xavier, Hörmann, Astrid, Ferrer, Pau, Kensy, Frank, Valero, Francisco
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4007594/
https://ncbi.nlm.nih.gov/pubmed/24606982
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-13-36
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