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Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase
BACKGROUND: In Pichia pastoris bioprocess engineering, classic approaches for clone selection and bioprocess optimization at small/micro scale using the promoter of the alcohol oxidase 1 gene (P(AOX1)), induced by methanol, present low reproducibility leading to high time and resource consumption. R...
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Format: | Artigo |
Sprache: | Inglês |
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BioMed Central
2014
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Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4007594/ https://ncbi.nlm.nih.gov/pubmed/24606982 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-13-36 |
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