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A Sequence-Independent Strategy for Detection and Cloning of Circular DNA Virus Genomes by Using Multiply Primed Rolling-Circle Amplification

The discovery of novel viruses has often been accomplished by using hybridization-based methods that necessitate the availability of a previously characterized virus genome probe or knowledge of the viral nucleotide sequence to construct consensus or degenerate PCR primers. In their natural replicat...

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Hlavní autoři: Rector, Annabel, Tachezy, Ruth, Van Ranst, Marc
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2004
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC400362/
https://ncbi.nlm.nih.gov/pubmed/15113879
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.78.10.4993-4998.2004
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