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Screening Target Specificity of siRNAs by Rapid Amplification of cDNA Ends (RACE) for Non-Sequenced Species

RNA interference (RNAi) is the process of sequence-specific posttranslational gene silencing triggered by double-stranded RNAs (dsRNAs). RNAi is a widely used approach for studying gene function. However, studies have shown that using siRNA can lead to off-target effects when the siRNA contains suff...

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Bibliografske podrobnosti
Main Authors: Sabirzhanov, Boris, Sabirzhanova, Inna B., Keifer, Joyce
Format: Artigo
Jezik:Inglês
Izdano: 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3992891/
https://ncbi.nlm.nih.gov/pubmed/21437668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12031-011-9514-6
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