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Calcium Phosphate Transfection of Primary Hippocampal Neurons
Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neu...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3989506/ https://ncbi.nlm.nih.gov/pubmed/24300106 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50808 |
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