Calcium Phosphate Transfection of Primary Hippocampal Neurons

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neu...

Full description

Saved in:
Bibliographic Details
Main Authors: Sun, Miao, Bernard, Laura P., DiBona, Victoria L., Wu, Qian, Zhang, Huaye
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2013
Subjects:
Neuroscience
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3989506/
https://ncbi.nlm.nih.gov/pubmed/24300106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50808
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items