Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases

Flow cytometric analysis of p38 mitogen-activated protein kinase (p38 MAPK) signaling cascade is optimally achieved by methanol permeabilization protocols. Such protocols suffer from the difficulties to accurately detect intracellular cytokines and surface epitopes of infrequent cell subpopulations,...

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Autors principals: Mavropoulos, Athanasios, Bogdanos, Dimitrios P., Liaskos, Christos, Orfanidou, Timoklia, Simopoulou, Theodora, Zafiriou, Efterpi, Sakkas, Lazaros I., Rigopoulou, Eirini I.
Format: Artigo
Idioma:Inglês
Publicat: Hindawi Publishing Corporation 2014
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Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3987974/
https://ncbi.nlm.nih.gov/pubmed/24741615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/671431
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