Flow Cytometric Detection of p38 MAPK Phosphorylation and Intracellular Cytokine Expression in Peripheral Blood Subpopulations from Patients with Autoimmune Rheumatic Diseases

Flow cytometric analysis of p38 mitogen-activated protein kinase (p38 MAPK) signaling cascade is optimally achieved by methanol permeabilization protocols. Such protocols suffer from the difficulties to accurately detect intracellular cytokines and surface epitopes of infrequent cell subpopulations,...

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Main Authors: Mavropoulos, Athanasios, Bogdanos, Dimitrios P., Liaskos, Christos, Orfanidou, Timoklia, Simopoulou, Theodora, Zafiriou, Efterpi, Sakkas, Lazaros I., Rigopoulou, Eirini I.
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3987974/
https://ncbi.nlm.nih.gov/pubmed/24741615
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/671431
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