Observation via one-dimensional 13Calpha NMR of local conformational substates in thermal unfolding equilibria of a synthetic analog of the GCN4 leucine zipper.

Synthesis of a 33-residue, capped leucine zipper analogous to that in GCN4 is reported. Histidine and arginine residues are mutated to lysine to reduce the unfolding temperature. CD and ultracentrifugation studies indicate that the molecule is a two-stranded coiled coil under benign conditions. Vers...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Lovett, E G, D'Avignon, D A, Holtzer, M E, Braswell, E H, Zhu, D, Holtzer, A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1996
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC39858/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8700835/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.5.1781
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