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Super-resolution optical DNA Mapping via DNA methyltransferase-directed click chemistry
We demonstrate an approach to optical DNA mapping, which enables near single-molecule characterization of whole bacteriophage genomes. Our approach uses a DNA methyltransferase enzyme to target labelling to specific sites and copper-catalysed azide-alkyne cycloaddition to couple a fluorophore to the...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3985630/ https://ncbi.nlm.nih.gov/pubmed/24452797 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt1406 |
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