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Library Generation by Gene Shuffling

This unit describes the process of gene shuffling (also known as sexual PCR). Gene shuffling is a facile method for the generation of sequence libraries containing the information from a family of related genes. Essentially, related genes are fragmented by DNase I digestion and reassembled by primer...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Meyer, Adam J., Ellefson, JaredW., Ellington, Andrew D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3983707/
https://ncbi.nlm.nih.gov/pubmed/24510437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb1512s105
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