A chemically cleavable biotinylated nucleotide: usefulness in the recovery of protein-DNA complexes from avidin affinity columns.

A biotinylated nucleotide analog containing a disulfide bond in the 12-atom linker joining biotin to the C-5 of the pyrimidine ring has been synthesized. This analog, Bio-SS-dUTP, is an efficient substrate for Escherichia coli DNA polymerase I. Bio-SS-dUTP supported DNA synthesis in a standard nick-...

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izdano v:	Proc Natl Acad Sci U S A
Principais autores:	Shimkus, M, Levy, J, Herman, T
Format:	Artigo
Jezik:	Inglês
Izdano:	National Academy of Sciences 1985
Teme:	Research Article
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397610/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3887407/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.9.2593
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