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A method for mapping intranuclear protein-DNA interactions and its application to a nuclease hypersensitive site.
We have devised a method for mapping sites on DNA within the nucleus that are protected against nuclease attack by interaction with bound protein or other factors. This "footprinting" method uses an end-labeled sequence-specific DNA probe, which is annealed to the DNA from nuclear digests...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3857580/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.8.2296 |
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