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Template-directed Chemical Ligation to Obtain 3′-3′ and 5′-5′ Phosphodiester DNA Linkages

Up to now, the direct ligation of two DNA fragments with opposite directions to obtain 3′-3′ or 5′-5′ phosphate ester bonds is still challenging. The only way to obtain DNA oligonucleotides containing a 3′-3′ or 5′-5′ inversion of polarity sites is based on professional DNA chemical synthesis. Herei...

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Autori principali: Chen, Haodong, Du, Feng, Chen, Gangyi, Streckenbach, Frank, Yasmeen, Afshan, Zhao, Yun, Tang, Zhuo
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3975322/
https://ncbi.nlm.nih.gov/pubmed/24699719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04595
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