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Simultaneous Detection of Antigen-Specific IgG- and IgM-Secreting Cells with a B Cell Fluorospot Assay

The traditional enzyme-linked immunospot (ELISpot) assay is the gold standard for the enumeration of antigen-specific B cells. Since B cell availability from biological samples is often limited, either because of sample size/volume or the need of performing multiple analyses on the same sample, the...

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Autors principals: Bonsignori, Mattia, Moody, M. Anthony
Format: Artigo
Idioma:Inglês
Publicat: MDPI 2012
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3972644/
https://ncbi.nlm.nih.gov/pubmed/24710360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells1010015
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