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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Autoantibodies, which are antibodies against self-antigens, are present in many disease states and can serve as markers for disease activity. The levels of autoantibodies to specific antigens are typically detected with the enzyme-linked immunosorbent assay (ELISA) technique. However, screening for...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Chruscinski, Andrzej, Huang, Flora Y. Y., Ulndreaj, Antigona, Chua, Conan, Fehlings, Michael, Rao, Vivek, Ross, Heather J., Levy, Gary A.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5092032/
https://ncbi.nlm.nih.gov/pubmed/27685156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54543
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