Cyclization of RNA 3'-terminal phosphate by cyclase from HeLa cells proceeds via formation of N(3')pp(5')A activated intermediate.

RNA 3'-terminal phosphate cyclase has been partially purified from HeLa cells. In the presence of ATP and Mg2+, cyclase preparations catalyze conversion of RNA 3'-terminal phosphate to the 2',3'-cyclic phosphodiester. The mechanism of 3'-phosphate cyclization was studied wit...

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Autores principales: Filipowicz, W, Strugala, K, Konarska, M, Shatkin, A J
Formato: Artigo
Lenguaje:Inglês
Publicado: 1985
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC397251/
https://ncbi.nlm.nih.gov/pubmed/2579395
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