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Genetic screens in human cells using the CRISPR/Cas9 system
The bacterial CRISPR/Cas9 system for genome editing has greatly expanded the toolbox for mammalian genetics, enabling the rapid generation of isogenic cell lines and mice with modified alleles. Here, we describe a pooled, loss-of-function genetic screening approach suitable for both positive and neg...
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| Main Authors: | , , , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
2013
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3972032/ https://ncbi.nlm.nih.gov/pubmed/24336569 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.1246981 |
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