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A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

The RNA polymerase gene of bacteriophage T7 has been cloned into the plasmid pBR322 under the inducible control of the lambda PL promoter. After induction, T7 RNA polymerase constitutes 20% of the soluble protein of Escherichia coli, a 200-fold increase over levels found in T7-infected cells. The ov...

詳細記述

保存先:
書誌詳細
主要な著者: Tabor, S, Richardson, C C
フォーマット: Artigo
言語:Inglês
出版事項: 1985
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC397196/
https://ncbi.nlm.nih.gov/pubmed/3156376
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