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A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
The RNA polymerase gene of bacteriophage T7 has been cloned into the plasmid pBR322 under the inducible control of the lambda PL promoter. After induction, T7 RNA polymerase constitutes 20% of the soluble protein of Escherichia coli, a 200-fold increase over levels found in T7-infected cells. The ov...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
1985
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC397196/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3156376/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.82.4.1074 |
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