Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity

The discovery of microRNAs (miRNAs), which are ∼21–23 nucleotides that can regulate targeted mRNA by transcript cleavage or protein translation suppression, has changed the landscape of biomedical field greatly. At present, Northern blot analysis based on radioisotopes is still the most popular meth...

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Bibliographic Details
Main Authors: Wu, Wei, Gong, Pengtao, Li, Jianhua, Yang, Ju, Zhang, Guocai, Li, He, Yang, Zhengtao, Zhang, Xichen
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 2014
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3964919/
https://ncbi.nlm.nih.gov/pubmed/24572812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.042150.113
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