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Tracking Cholesterol/Sphingomyelin-Rich Membrane Domains with the Ostreolysin A-mCherry Protein

Ostreolysin A (OlyA) is an ∼15-kDa protein that has been shown to bind selectively to membranes rich in cholesterol and sphingomyelin. In this study, we investigated whether OlyA fluorescently tagged at the C-terminal with mCherry (OlyA-mCherry) labels cholesterol/sphingomyelin domains in artificial...

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Detalhes bibliográficos
Main Authors: Skočaj, Matej, Resnik, Nataša, Grundner, Maja, Ota, Katja, Rojko, Nejc, Hodnik, Vesna, Anderluh, Gregor, Sobota, Andrzej, Maček, Peter, Veranič, Peter, Sepčić, Kristina
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3963934/
https://ncbi.nlm.nih.gov/pubmed/24664106
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0092783
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