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Disulfide bond generation in mammalian blood serum; detection and purification of Quiescin-sulfhydryl oxidase

A sensitive new plate reader assay has been developed showing that adult mammalian blood serum contains circulating soluble sulfhydryl oxidase activity that can introduce disulfide bonds into reduced proteins with the reduction of oxygen to hydrogen peroxide. The activity was purified 5000-fold to &...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Israel, Benjamin A., Jiang, Lingxi, Gannon, Shawn A., Thorpe, Colin
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3960832/
https://ncbi.nlm.nih.gov/pubmed/24468475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2014.01.020
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