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Characterization of a Novel Melt Curve by Use of the Roche LightCycler HSV 1/2 Analyte-Specific Reagent Real-Time PCR Assay: Frequencies of This Novel (Low) Melt Curve and Commonly Encountered (Intermediate) Melt Curves

We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%,...

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Bibliografiske detaljer
Main Authors: Boyanton, Bobby L., Almradi, Amro, Espy, Mark J., Prada, Anne E., Gibson, John P., Pritt, Bobbi S.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3957778/
https://ncbi.nlm.nih.gov/pubmed/24352998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.03062-13
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