Sequencing and Resolution of Amplified Herpes Simplex Virus DNA with Intermediate Melting Curves as Genotype 1 or 2 by LightCycler PCR Assay

DNA from 101 specimens containing herpes simplex virus (HSV) produced atypical intermediate melting curves compared with those expected for HSV type 1 or HSV type 2 subsequent to real-time PCR. Nucleic acid sequence analysis of amplified target DNA revealed 1- or 3-bp polymorphisms in the probe regi...

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Detalhes bibliográficos
Main Authors: Issa, Nicolas C., Espy, Mark J., Uhl, James R., Smith, Thomas F.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1081363/
https://ncbi.nlm.nih.gov/pubmed/15815006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.4.1843-1845.2005
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