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Un-induced high-yield bacterial expression of fluorescent proteins

Here we introduce a fast, cost-effective, and highly efficient method for production of soluble fluorescent proteins from bacteria. The method does not require optimization, and does not utilize IPTG induction. The method relies on un-induced expression in the BL21-gold (DE3) strain of E. coli and y...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Sarabipour, Sarvenaz, King, Christopher, Hristova, Kalina
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3957324/
https://ncbi.nlm.nih.gov/pubmed/24378720
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.12.027
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