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Un-induced high-yield bacterial expression of fluorescent proteins
Here we introduce a fast, cost-effective, and highly efficient method for production of soluble fluorescent proteins from bacteria. The method does not require optimization, and does not utilize IPTG induction. The method relies on un-induced expression in the BL21-gold (DE3) strain of E. coli and y...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3957324/ https://ncbi.nlm.nih.gov/pubmed/24378720 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.12.027 |
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