False positive staining in the TUNEL assay to detect apoptosis in liver and intestine is caused by endogenous nucleases and inhibited by diethyl pyrocarbonate.

BACKGROUND: The terminal transferase uridyl nick end labelling (TUNEL) assay allows the easy demonstration of cell death as a result of apoptosis. However, when this assay is applied to liver tissue, the number of TUNEL positive cells is dependent on the time of incubation with proteinase K. AIM: To...

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Pubblicato in:	Mol Pathol
Autori principali:	Stähelin, B J, Marti, U, Solioz, M, Zimmermann, H, Reichen, J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMJ Publishing Group 1998
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC395637/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9893746/ https://ncbi.nlm.nih.govhttps://doi.org/10.1136/mp.51.4.204
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