A general assay for antibody catalysis using acridone as a fluorescent tag.
A simple and highly sensitive catalysis assay is demonstrated based on analyzing reactions with acridonetagged compounds by thin-layer chromatography. As little as 1 pmol of product is readily visualized by its blue fluorescence under UV illumination and identified by its retention factor (Rf). Each...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1996
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC39521/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8633050/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.9.4251 |
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