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A general assay for antibody catalysis using acridone as a fluorescent tag.

A simple and highly sensitive catalysis assay is demonstrated based on analyzing reactions with acridonetagged compounds by thin-layer chromatography. As little as 1 pmol of product is readily visualized by its blue fluorescence under UV illumination and identified by its retention factor (Rf). Each...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Reymond, J L, Koch, T, Schröer, J, Tierney, E
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1996
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC39521/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8633050/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.9.4251
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