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Syn5 RNA polymerase synthesizes precise run-off RNA products
The enzyme predominantly used for in vitro run-off RNA synthesis is bacteriophage T7 RNA polymerase. T7 RNA polymerase synthesizes, in addition to run-off products of precise length, transcripts with an additional non-base-paired nucleotide at the 3′-terminus (N + 1 product). This contaminating prod...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2014
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3950665/ https://ncbi.nlm.nih.gov/pubmed/24285303 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt1193 |
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