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Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

Approaches that depend on directed evolution require reliable methods to generate DNA diversity so that mutant libraries can focus on specific target regions. We took advantage of the high frequency of homologous DNA recombination in Saccharomyces cerevisiae to develop a strategy for domain mutagene...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς: Gonzalez-Perez, David, Molina-Espeja, Patricia, Garcia-Ruiz, Eva, Alcalde, Miguel
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: Public Library of Science 2014
Θέματα:
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3948698/
https://ncbi.nlm.nih.gov/pubmed/24614282
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0090919
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