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Patch-Clamp Recordings and Calcium Imaging Followed by Single-Cell PCR Reveal the Developmental Profile of 13 Genes in iPSC-Derived Human Neurons

Molecular genetic studies are typically performed on homogenized biological samples, resulting in contamination from non-neuronal cells. To improve expression profiling of neurons we combined patch recordings with single-cell PCR. Two iPSC lines (healthy subject and 22q11.2 deletion), were different...

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Detalhes bibliográficos
Main Authors: Belinsky, Glenn S., Rich, Matthew T., Sirois, Carissa L., Short, Shaina M., Pedrosa, Erika, Lachman, Herbert M., Antic, Srdjan D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3947234/
https://ncbi.nlm.nih.gov/pubmed/24157591
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.scr.2013.09.014
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