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Patch-Clamp Recordings and Calcium Imaging Followed by Single-Cell PCR Reveal the Developmental Profile of 13 Genes in iPSC-Derived Human Neurons
Molecular genetic studies are typically performed on homogenized biological samples, resulting in contamination from non-neuronal cells. To improve expression profiling of neurons we combined patch recordings with single-cell PCR. Two iPSC lines (healthy subject and 22q11.2 deletion), were different...
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Main Authors: | , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3947234/ https://ncbi.nlm.nih.gov/pubmed/24157591 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.scr.2013.09.014 |
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