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Expanding Proteome Coverage with Orthogonal-specificity α-Lytic Proteases
Bottom-up proteomics studies traditionally involve proteome digestion with a single protease, trypsin. However, trypsin alone does not generate peptides that encompass the entire proteome. Alternative proteases have been explored, but most have specificity for charged amino acid side chains. Therefo...
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| Główni autorzy: | , , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
The American Society for Biochemistry and Molecular Biology
2014
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3945911/ https://ncbi.nlm.nih.gov/pubmed/24425750 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M113.034710 |
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