Expanding Proteome Coverage with Orthogonal-specificity α-Lytic Proteases

Bottom-up proteomics studies traditionally involve proteome digestion with a single protease, trypsin. However, trypsin alone does not generate peptides that encompass the entire proteome. Alternative proteases have been explored, but most have specificity for charged amino acid side chains. Therefo...

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Detalhes bibliográficos
Main Authors: Meyer, Jesse G., Kim, Sangtae, Maltby, David A., Ghassemian, Majid, Bandeira, Nuno, Komives, Elizabeth A.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2014
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3945911/
https://ncbi.nlm.nih.gov/pubmed/24425750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M113.034710
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