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Comprehensive Cysteine-scanning Mutagenesis Reveals Claudin-2 Pore-lining Residues with Different Intrapore Locations

The first extracellular loop (ECL1) of claudins forms paracellular pores in the tight junction that determine ion permselectivity. We aimed to map the pore-lining residues of claudin-2 by comprehensive cysteine-scanning mutagenesis of ECL1. We screened 45 cysteine mutations within the ECL1 by expres...

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Bibliografiske detaljer
Main Authors: Li, Jiahua, Zhuo, Min, Pei, Lei, Rajagopal, Madhumitha, Yu, Alan S. L.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Biochemistry and Molecular Biology 2014
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3945313/
https://ncbi.nlm.nih.gov/pubmed/24436330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.536888
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