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Quantitative analysis of deadenylation-independent mRNA decay by a modified MBRACE assay

Endonuclease cleavage is the rate-limiting step in the decay of nonsense-containing human β-globin mRNA in erythroid cells. The 5’--truncated intermediates thus generated are polyadenylated and more stable than the parent mRNA. Northern blotting is commonly used to measure the decay rate of full-len...

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Detalhes bibliográficos
Main Authors: Dougherty, Julie A., Mascarenhas, Roshan, Schoenberg, Daniel R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3942805/
https://ncbi.nlm.nih.gov/pubmed/24590802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-971-0_28
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