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Quantitative analysis of deadenylation-independent mRNA decay by a modified MBRACE assay
Endonuclease cleavage is the rate-limiting step in the decay of nonsense-containing human β-globin mRNA in erythroid cells. The 5’--truncated intermediates thus generated are polyadenylated and more stable than the parent mRNA. Northern blotting is commonly used to measure the decay rate of full-len...
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| Asıl Yazarlar: | , , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2014
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3942805/ https://ncbi.nlm.nih.gov/pubmed/24590802 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-971-0_28 |
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