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Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.

Biotin-labelled DNA probes, prepared by nick-translation in the presence of biotinylated analogs of TTP, are hybridized to DNA or RNA immobilized on nitrocellulose filters. After removal of residual probe, the filters are incubated for 2--5 min with a preformed complex made with avidin-DH (or strept...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Leary, J J, Brigati, D J, Ward, D C
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394197/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6191328/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.13.4045
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