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Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.

Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from...

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Detalhes bibliográficos
Main Authors: Biggin, M D, Gibson, T J, Hong, G F
Formato: Artigo
Idioma:Inglês
Publicado em: 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC394179/
https://ncbi.nlm.nih.gov/pubmed/6575390
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