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Identification of a 31,500 molecular weight islet cell protease as cathepsin B.

A method for the preparation of a radioisotopically labeled active-site directed reagent for proteases (125I-Tyr-Ala-Lys-ArgCH2Cl) is described, and an example of its use as a sensitive method for identifying trypsin-like proteases is provided. This high specific activity reagent was then used in an...

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Библиографические подробности
Опубликовано в: :Proc Natl Acad Sci U S A
Главные авторы: Docherty, K, Carroll, R, Steiner, D F
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 1983
Предметы:
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC394017/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6344078/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.11.3245
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