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Mapping of single-stranded regions in duplex DNA at the sequence level: single-strand-specific cytosine methylation in RNA polymerase-promoter complexes.

A method based on the differential rate of cytosine methylation in single- and double-stranded nucleic acids by dimethyl sulfate [Peattie, D.A. & Gilbert, W. (1980) Proc. Natl. Acad. Sci. USA 77, 4679-4682] has been developed for probing unpaired cytosines in DNA and DNA-protein complexes at the seq...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Kirkegaard, K, Buc, H, Spassky, A, Wang, J C
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393862/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6573669/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.9.2544
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