Mapping of single-stranded regions in duplex DNA at the sequence level: single-strand-specific cytosine methylation in RNA polymerase-promoter complexes.

A method based on the differential rate of cytosine methylation in single- and double-stranded nucleic acids by dimethyl sulfate [Peattie, D.A. & Gilbert, W. (1980) Proc. Natl. Acad. Sci. USA 77, 4679-4682] has been developed for probing unpaired cytosines in DNA and DNA-protein complexes at the...

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Detalles Bibliográficos
Main Authors: Kirkegaard, K, Buc, H, Spassky, A, Wang, J C
Formato: Artigo
Idioma:Inglês
Publicado: 1983
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC393862/
https://ncbi.nlm.nih.gov/pubmed/6573669
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