Chimeric plasmid that replicates autonomously in both Escherichia coli and Neurospora crassa.

A hybrid pBR322 plasmid (designated pDV1001) containing two functional Escherichia coli antibiotic resistance genes (kanr and camr) and a qa-2+ gene from Neurospora crassa transforms N. crassa qa-2- mutants to qa-2+ with a frequency of ca. 5 X 10(-5) per regenerated spheroplast (ca. 100 transformant...

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Bibliographic Details
Main Authors: Hughes, K, Case, M E, Geever, R, Vapnek, D, Giles, N H
Format: Artigo
Language:Inglês
Published: 1983
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC393526/
https://ncbi.nlm.nih.gov/pubmed/6302666
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