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Chimeric plasmid that replicates autonomously in both Escherichia coli and Neurospora crassa.
A hybrid pBR322 plasmid (designated pDV1001) containing two functional Escherichia coli antibiotic resistance genes (kanr and camr) and a qa-2+ gene from Neurospora crassa transforms N. crassa qa-2- mutants to qa-2+ with a frequency of ca. 5 X 10(-5) per regenerated spheroplast (ca. 100 transformant...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1983
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393526/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6302666/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.4.1053 |
| Oznake: |
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