Procedure for rapid isolation of photosynthetic reaction centers using cytochrome c affinity chromatography
Horse heart cytochrome c linked to Sepharose 4B is used to purify reaction centers from Rhodopseudomonas sphaeroides R-26. This procedure allows for an initial recovery of 80-90% of the bacterial reaction centers present in chromatophore membranes. High purity reaction centers (A(280)/A(802) < 1.30)...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1983
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393443/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16578765/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.3.683 |
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