Procedure for rapid isolation of photosynthetic reaction centers using cytochrome c affinity chromatography

Horse heart cytochrome c linked to Sepharose 4B is used to purify reaction centers from Rhodopseudomonas sphaeroides R-26. This procedure allows for an initial recovery of 80-90% of the bacterial reaction centers present in chromatophore membranes. High purity reaction centers (A(280)/A(802) < 1.30)...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Brudvig, G. W., Worland, S. T., Sauer, K.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1983
Assuntos:	Biological Sciences: Biochemistry
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393443/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16578765/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.3.683
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