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Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans.

Glucokinase (ATP:D-glucose 6-phosphotransferase, EC 2.7.1.2) from rat islets of Langerhans was partially purified by chromatography on DEAE-Cibacron blue F3GA agarose. The enzyme eluted in two separate peaks. Sigmoidal rate dependence was found with respect to glucose (Hill coefficient = 1.5) for bo...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Meglasson, M D, Burch, P T, Berner, D K, Najafi, H, Vogin, A P, Matschinsky, F M
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393314/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6337376/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.1.85
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