Chromatographic resolution and kinetic characterization of glucokinase from islets of Langerhans.

Glucokinase (ATP:D-glucose 6-phosphotransferase, EC 2.7.1.2) from rat islets of Langerhans was partially purified by chromatography on DEAE-Cibacron blue F3GA agarose. The enzyme eluted in two separate peaks. Sigmoidal rate dependence was found with respect to glucose (Hill coefficient = 1.5) for bo...

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Détails bibliographiques
Auteurs principaux: Meglasson, M D, Burch, P T, Berner, D K, Najafi, H, Vogin, A P, Matschinsky, F M
Format: Artigo
Langue:Inglês
Publié: 1983
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC393314/
https://ncbi.nlm.nih.gov/pubmed/6337376
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